Untargeted Metabolomics Data Analysis

Raw data obtained from the LC-MS analysis were converted to mzXML centroid files using Shimadzu LabSolutions Postrun Analysis. The files were imported into Mzmine 2.53 for data processing.^{37 (link)} The resulting quantification tables were uploaded to MetaboAnalyst and subjected to RM two-way ANOVA.^{38 (link)} The exported quantification table for GNPS and the MS2 spectra were uploaded to Feature Based Molecular Networking on the GNPS platform^{39 (link),40 (link)} using the default settings. The resulting network was visualized in Cytoscape 3.4.0. Details regarding data processing are depicted in the supplementary methods.

Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link: Access Free Full Text.

Jansma J., Thome N.U., Schwalbe M., Chatziioannou A.C., Elsayed S.S., van Wezel G.P., van den Abbeele P., van Hemert S, & El Aidy S. (2023). Dynamic effects of probiotic formula ecologic®825 on human small intestinal ileostoma microbiota: a network theory approach. Gut Microbes, 15(1), 2232506.

Publication 2023

Postrun Analysis

Anova

Corresponding Organization : University of Groningen

Other organizations : Leiden University, Netherlands Institute of Ecology, Winclove Probiotics (Netherlands)

Top 5 similar protocols

Variable analysis

independent variables

Not explicitly mentioned

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!