Bacterial Challenge and ZOI Assays

The following bacterial strains were used for bacterial challenge and zone of inhibition (ZOI) assays: Micrococcus luteus (lysodeikticus) (ATCC, no. 4698), live Staphylococcus aureus [strain PIG1; Liu et al., 2005 (link))], and Escherichia coli (OP50, NCBI:txid637912). ZOI assays were also performed with Saccharomyces cerevisiae (baker’s active dried yeast from MP Biomedicals, Solon, OH, USA).

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Morejon B, & Michel K. (2023). A zone-of-inhibition assay to screen for humoral antimicrobial activity in mosquito hemolymph. Frontiers in Cellular and Infection Microbiology, 13, 891577.

Publication 2023

Saccharomyces cerevisiae

Assays Bacterial Dried yeast Escherichia coli Inhibition Micrococcus luteus Saccharomyces cerevisiae Solon Staphylococcus aureus Strain

Corresponding Organization : Kansas State University

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Variable analysis

independent variables

Bacterial strains used for bacterial challenge and zone of inhibition (ZOI) assays: Micrococcus luteus (lysodeikticus) (ATCC, no. 4698), live Staphylococcus aureus [strain PIG1; Liu et al., 2005 (link)], Escherichia coli (OP50, NCBI:txid637912), and Saccharomyces cerevisiae (baker's active dried yeast from MP Biomedicals, Solon, OH, USA)

dependent variables

Zone of inhibition (ZOI) measured as the outcome of the assays

control variables

Not explicitly mentioned

positive controls

Not specified

negative controls

Not specified

Annotations

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