Flavonolignan 2 Modulates Mitochondrial Membrane Potential and ROS in CCRF-CEM Cells

The modification of the MMP as well as the ROS levels after the application of BAL, and flavonolignan 2 at ¼ IC₅₀, ½ IC₅₀, IC₅₀, and 2 × IC₅₀ to CCRF-CEM cells (1 × 10⁶) was performed by flow cytometry [45 (link)]. DMSO was used as solvent control while valinomycin served as a positive control. The cells were incubated for 24 h and further stained for 30 min with JC-1 [45 (link)] and measured (1 × 10⁴ cells) in an LSR-Fortessa FACS analyzer (Becton–Dickinson) [45 (link)]. For ROS determination, CCEF-CEM cells were similarly treated BAL, the flavonolignan 2, hydrogen peroxide (H₂O₂; positive control), DMSO (solvent control) as in the MMP analysis, then incubated for 24 h. They were further stained with H₂DCFH-DA and measured (1 × 10⁴ cells) in an LSR-Fortessa FACS analyzer [40 (link), 51 (link), 52 (link)].