The scaffolds were produced using an NF-103 (MESS, Fukuoka, Japan) electrospinning device under the conditions previously described [24 (link)]. PU-gelatin scaffolds were made at a collector rotation speed of 300 rpm, a voltage of 18.5–19 kV, and a solution flow rate of 1.1–1.2 mL/h. The electrospinning solution was composed of 3% Tecoflex EG-80A polyurethane (batch 01016300734, Lubrizol Advanced Materials, Beveren, Belgium) (w/v) and 15% gelatin from porcine skin (Sigma № G2500, Saint Louis, MI, USA) (w (gelatin)/w (PU)) in 1,1,1,3,3,3-hexafluoroisopropanol (Sigma, Saint Louis, MI, USA). After preparation, scaffolds were dried in a fore vacuum for at least 3 h to remove residual solvent before being stored in sealed zip-lock bags.
Flat films (100–120 µm thick) were obtained by casting a glass slide with 3% Tecoflex EG-80A polyurethane solution (w/v) in 1,1,1,3,3,3-hexafluoroisopropanol at room temperature (23–25 °C). After 3 days of incubation, fabricated flat films at room temperature were additionally dried using a fore vacuum to remove the residual solvent and stored in sealed zip-lock containers.
Flat films (100–120 µm thick) were obtained by casting a glass slide with 3% Tecoflex EG-80A polyurethane solution (w/v) in 1,1,1,3,3,3-hexafluoroisopropanol at room temperature (23–25 °C). After 3 days of incubation, fabricated flat films at room temperature were additionally dried using a fore vacuum to remove the residual solvent and stored in sealed zip-lock containers.
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