Synthesis and Characterization of Antibody-Ligand Complexes
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Other organizations : Arizona State University
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Variable analysis
- Thiolated streptavidin with an average of 2.5 thiols per tetramer
- Anti-DNP antibody (mouse monoclonal IgE antibody)
- Wild-type streptavidin
- Binding affinities of all three antibodies (anti-HIV, anti-Ebola, and anti-DNP) measured by surface plasmon resonance
- RGD peptide purchased from Peptides International
- Peptide ligands for the anti-HIV antibody and the anti-Ebola antibody synthesized by CPC Scientific with a purity >95%
- DNP and biotin disulfides synthesized in the laboratory and reduced for 2 h before use by an immobilized (Tris[2-carboxyethyl] phosphine hydrochloride) disulfide reducing gel from Thermo Scientific
- Anti-HIV antibody (Anti-HIV1 p17 antibody [32/1.24.89]) and all of the isotype controls obtained from Abcam
- Anti-Ebola antibody cultured from plants
- Ag/AgCl reference electrodes salt-bridged by 3 M KCl or 10 mM KCl prepared as described previously
- Anti-Ebola antibody and the corresponding monomeric Fab fragment prepared and purified as described in the SI Appendix
- Anti-DNP antibody (mouse monoclonal IgE antibody)
- Isotype controls for the anti-HIV and anti-Ebola antibodies
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