Unilateral Ureteral Obstruction and Diabetic Kidney Disease

For the unilateral ureteral obstruction (UUO) model, C57BL/6 J male mice (n = 4–5, age, 8 weeks) were anesthetized under isoflurane and the left ureter was accessed dorsally and ligated (or not, for sham-operated controls) [11 (link)]; blood was collected from the abdominal aorta 7 days after ligation. For streptozocin (STZ)-induced diabetic mice, C57BL/6 J male mice (n = 5–6, age, 8 weeks) received STZ (50 mg/kg, Sigma-Aldrich, St. Louis, MO, USA) or citrate buffer (pH 4.5) intraperitoneally once daily for 5 consecutive days; 11 weeks after the last dose of STZ, blood was collected from the abdominal femoral artery. The urinary protein level in each mouse was measured by using Micro TP-Test photometric (FujiFilm Wako Pure Chemical Corporation, Osaka, Japan) according to the manufacturer’s protocol. Blood glucose concentration was measured by using a LabAssay Glucose kit (FujiFilm Wako Pure Chemical Corporation) according to manufacturer’s protocol.