Fungal DNA Extraction and Sequencing

DNA was extracted from the cultures grown on PDA for 7 days using the Plant Genomic DNA Kit (DP305, TIANGEN Biotech, Beijing, China). Polymerase chain reaction (PCR) amplifications of the internal transcribed spacer (ITS), beta-tubulin (BenA), calmodulin (CaM) and RNA polymerase II second largest subunit (RPB2) gene regions were conducted with the routine methods [21 (link),22 (link),23 (link),24 (link)]. The products were purified and subject to sequencing on an ABI 3730 DNA Sequencer (Applied Biosystems). Although the ITS region is proposed as the universal DNA barcode for fungi, it is not sufficient to distinguish species of Aspergillus. The ITS sequences provided in this study might be helpful for other researchers in case of need.