Citrullinated histone H3 (H3Cit) was quantified with a slight modification of the ELISA previously described by Thalin et al. (19 (link)) by using Cell Death detection kit without streptavidin-precoated wells. The optical densities (ODs) were measured at a wavelength of 450 nm with a reference correction wavelength at 620 nm using a microplate photometer (Infinite® 200 PRO NanoQuant Multimode Microplate Reader, Tecan).
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