Evaluating Safety of CS-FA-MBZ Implants in Mice

For evaluating the safety of the subcutaneous CS-FA-MBZ implants, 20 healthy mice were involved and randomly divided into four groups (n = 5) including (1) Control, (2) MBZ, (3) CS-FA, and (4) CS-FA-MBZ implants according to the “Tumor-bearing mice grouping and therapeutic approaches” section. The mice were monitored for general appearance and behavioral parameters for 30 days. They were under close monitored for any signs of toxicity and behavioral changes including weakness, salivation, anorexia, diarrhea, aggressiveness, eyes and ears discharge, noisy breathing, activity, convulsion, cachexia, pain, or any signs of illness in each group for 30 days [71 (link)]. On the 30th day, the mice were sacrificed and blood urea nitrogen (BUN), creatinine (Cr), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels were measured in the discarded serums [72 (link)]. In addition, lungs, kidneys, liver, and spleen were harvested and fixed, processed, and H&E stained. Histological photographs were obtained using a digital light microscope (Olympus, Japan).