Cytotoxicity Evaluation of Anti-cancer Drugs

Cells were seeded into 96-well plates at a density of 5 × 10³ cells per well. After one day of growth, the cells were treated with different concentrations of Etoposid (VP – 16) (Selleck, USA) 5-Fluorouracil (5 – FU) (Selleck, USA), Methotrexate (MTX) (Selleck, USA), Paclitaxel (TAXOL) (Selleck, USA), or ActD (Selleck, USA) and incubated at 37°C for 72 hours. Cell viability was detected by CCK – 8 viability assay at 72 hours after drug treatment [9 (link)]. The absorbance at 490 nm was measured with a Varioskan Flash microplate reader (Thermo Fish Scientific, Waltham, MA, USA) and the results were calculated using SPSS software.
The concentrations of drugs used in MTX-resistant cells were listed below:
VP-16:0.05, 0.1, 0.2, 0.5, 1, 2, 5, 10, 20 ng/ml;
5-FU:0.05, 0.1, 0.5, 1, 5, 10, 20, 50 μg/ml;
TAXOL:0.06, 0.1, 0.3, 0.6, 1, 3, 6, 10, 30 nM;
MTX:1, 4, 10, 40, 100, 400, 1000 μM;
ActD:0.1, 0.4, 0.8, 1, 4, 8, 10, 40 μg/ml.
The concentrations of drugs used in original cells were listed below:
VP-16:0.005, 0.01, 0.05, 0.1, 0.5, 1, 2 ng/ml;
5-FU:0.05, 0.1, 0.5, 1, 5, 10, 20 μg/ml;
TAXOL:0.06, 0.1, 0.3, 0.6, 1, 3, 6, 10, 30 nM;
MTX:0.01, 0.04, 0.1, 0.4, 1, 4, 10, 40 μM;
ActD:0.1, 0.4, 0.8, 1, 4, 8, 10, 40 μg/ml.
(Note: It is converted into a uniform concentration unit for subsequent statistics)