qPCR analyses were conducted as described previously (74 (link)). Briefly, total cellular RNA isolated using the ISOLATE II RNA Mini Kit was subject to reverse transcription using qScript cDNA Supermix (Quantabio). Reactions (20 μL) were prepared on ice using SensiFAST SYBR Hi-ROX Kit (Bioline) reagents in combination with the primers listed in SI Appendix, Table S6 , and cycling was performed for 40 cycles at 95 °C (15 s) and at 60 °C (1 min). The 2−ΔΔCT method was used to calculate the relative gene expression levels in comparison to the β-actin housekeeping control.
Protocol detail
Quantitative PCR Analysis Protocol
Partial Protocol Preview
This section provides a glimpse into the protocol.
The remaining content is hidden due to licensing restrictions, but the full text is available at the following link:
Access Free Full Text.
Corresponding Organization :
Other organizations : Chinese Academy of Sciences, University of Science and Technology of China, Center for Excellence in Molecular Cell Science, Henan Provincial People's Hospital, Zhengzhou University, University of Newcastle Australia, Shanxi Provincial Cancer Hospital
Top 5 similar protocols
Protocol cited in 1 other protocol
Variable analysis
independent variables
- None explicitly mentioned
- Relative gene expression levels in comparison to the β-actin housekeeping control
- β-actin housekeeping gene
- Positive control: None mentioned
- Negative control: None mentioned
Annotations
Based on most similar protocols
Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.
Sign up for free or login to display all annotations
As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!