1-[4-(2-methoxyphenyl) piperaznyl]-3-(1-naphthyloxy) propan-2-ol (naftopidil) (PubChem CID: 4418) (Asahi Kasei Pharma Co., Tokyo, Japan) was dissolved in 1% dimethyl sulfoxide (PubChem CID: 679) (Wako, Osaka, Japan) in Krebs solution. Tamsulosin and silodosin were dissolved in Krebs solution. The concentration of naftopidil and tamsulosin used were 100μM, and silodosin used was 30μM. Since naftopidil (100μM) was effective in modulating synaptic transmission in superficial dorsal horn neurons of rodent spinal cord slices [15 (link)], naftopidil was used at 100μM in the present study. Although, we used same concentration of tamsulosin (100μM), we could not prepare the same concentration of silodosin. However, the affinity of the receptor of silodosin is 100 times higher than naftopidil [21 (link)], so we considered 30μM silodosin is sufficient concentration. All drugs were applied by perfusion sequentially in a single cell with wash-out periods via a 3-way stopcock without changes in the perfusion rate or temperature. The application schedule is summarized in Fig. 1B .
Protocol detail
In Vitro Evaluation of Selective Alpha-1 Antagonists
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Cell
Dimethyl sulfoxide
Dorsal horn of spinal cord
Drugs
Krebs solution
Naftopidil
Neurons
Perfusion
Rodent
Silodosin
Synaptic transmission
Tamsulosin
Corresponding Organization :
Other organizations : University of Toyama, Asahi Kasei (Japan), Kyushu University
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Variable analysis
independent variables
- Naftopidil (100μM)
- Tamsulosin (100μM)
- Silodosin (30μM)
- Synaptic transmission in superficial dorsal horn neurons of rodent spinal cord slices
- Perfusion rate
- Temperature
- Positive control: Naftopidil (100μM) was used as it was previously shown to be effective in modulating synaptic transmission in superficial dorsal horn neurons of rodent spinal cord slices.
- Negative control: Not explicitly mentioned.
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