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CRISPR-Cas9 Gene Disruption in Xenopus

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Teekakirikul P., Zhu W., Xu X., Young C.B., Tan T., Smith A.M., Wang C., Peterson K.A., Gabriel G.C., Ho S., Sheng Y., Moreau de Bellaing A., Sonnenberg D.A., Lin J.H., Fotiou E., Tenin G., Wang M.X., Wu Y.L., Feinstein T., Devine W., Gou H., Bais A.S., Glennon B.J., Zahid M., Wong T.C., Ahmad F., Rynkiewicz M.J., Lehman W.J., Keavney B., Alastalo T.P., Freckmann M.L., Orwig K., Murray S., Ware S.M., Zhao H., Feingold B, & Lo C.W. (2022). Genetic resiliency associated with dominant lethal TPM1 mutation causing atrial septal defect with high heritability. Cell Reports Medicine, 3(2), 100501.
Publication 2022
DR274 vector MAXIscript T7 transcription kit Cas9 protein
Assay Cas9 protein Cell Crispr Digestion Edema Embryos Gene Genomic Heart rate Microscope Oligonucleotides Transcription Vector Western blot

Corresponding Organization : University of Pittsburgh

Other organizations : Chinese University of Hong Kong, Indiana University – Purdue University Indianapolis, Jackson Laboratory, Magee-Womens Research Institute, University of Manchester, BGI Group (China), University of Iowa, Boston University, Blueprint Medicines (United States), Royal North Shore Hospital, Children's Hospital of Pittsburgh

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