All immunohistochemical staining procedures were performed as described previously (23 (link)). Briefly, the tissue sections were incubated with 3% hydrogen peroxide to inhibit endogenous peroxidase. After that, the slices were incubated with rabbit polyclonal anti-NF-kB (Abcam, Egypt) and rabbit polyclonal anti-COX2 (Abcam, Egypt) antibodies overnight at 4 °C. Diaminobenzidine was used for the demonstration of immune reaction. The immune reactivity for NF-kB and COX-2 was Semi quantitatively assessed in ten random high-power fields (HPF), according to the percentage of positive cells in the high- power field, as described by Hassan et al. (24 (link)). A semi-quantitative scale graded from 0 to 3 was used in which 0, no staining; 1, positive staining in < 30% of cells per HPF; 2, positive staining in 30-70% of cells per HPF; or 3, positive staining in > 70% of cells per HPF.