The main goal of preparing sections of the hippocampus and staining them was to investigate possible changes in brain tissue and to quantitatively examine the amyloid fibrils formed in the absence and presence of silibinin and trans-chalcone in each section. Five tissue sections in each group were randomly examined microscopically. Since we observed that silibinin performed better than trans-chalcone in the initial tests, both DG and CA1 regions were checked for the former and DG for the latter.
Histological Analysis of Silibinin and trans-Chalcone Effects on Hippocampal Amyloid Fibrils
The main goal of preparing sections of the hippocampus and staining them was to investigate possible changes in brain tissue and to quantitatively examine the amyloid fibrils formed in the absence and presence of silibinin and trans-chalcone in each section. Five tissue sections in each group were randomly examined microscopically. Since we observed that silibinin performed better than trans-chalcone in the initial tests, both DG and CA1 regions were checked for the former and DG for the latter.
Corresponding Organization :
Other organizations : Islamic Azad University, Science and Research Branch, University of Tehran, Tehran University of Medical Sciences
Variable analysis
- Presence or absence of silibinin
- Presence or absence of trans-chalcone
- Changes in brain tissue
- Quantitative examination of amyloid fibrils formed
- Tissue sections were prepared using the same protocol (fixation, dehydration, embedding, and sectioning)
- Staining methods (hematoxylin-eosin and thioflavin S) were applied consistently
- Positive control: Samples stained with hematoxylin-eosin
- Negative control: Samples stained with thioflavin S
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