Immunohistochemical Analysis of Tumor Markers

We performed IHC in paraffin-embedded samples obtained from the CGGA sample bank. IHC analysis with AIF1 (Proteintech, 10904-1-AP, 1:500), TNF (Abcam, ab270264,1:150), CD163 (Abcam, ab189915, 1:500), and TIM3 (Abcam, ab241332, 1:500) antibodies were conducted according to our previous procedures [24 (link), 25 (link)]. The protein expression levels were evaluated independently by two experienced pathologists and the scoring criteria refer to our published article [25 (link)].

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Zhang Y., Ma W., Fan W., Ren C., Xu J., Zeng F., Bao Z., Jiang T, & Zhao Z. (2021). Comprehensive transcriptomic characterization reveals core genes and module associated with immunological changes via 1619 samples of brain glioma. Cell Death & Disease, 12(12), 1140.

Publication 2021

ab270264 ab189915 ab241332

Antibodies Cd163 Embedded paraffin Pathologists Protein Tim3

Corresponding Organization :

Other organizations : Beijing Institute of Neurosurgery, Capital Medical University, Beijing Sanbo Brain Hospital, Harbin Medical University, Second Affiliated Hospital of Harbin Medical University

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Variable analysis

independent variables

IHC analysis with AIF1 (Proteintech, 10904-1-AP, 1:500)
IHC analysis with TNF (Abcam, ab270264,1:150)
IHC analysis with CD163 (Abcam, ab189915, 1:500)
IHC analysis with TIM3 (Abcam, ab241332, 1:500)

dependent variables

Protein expression levels

control variables

Paraffin-embedded samples obtained from the CGGA sample bank
Scoring criteria referred to the published article [25]

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