Protocol detail

Pharmaceutical Reference Standard Protocol

Find Similar Protocols

In the study following materials were used: pharmaceutical secondary standards (Certified Reference Material) of acetylsalicylic acid, salicylic acid and glycine (Fluka). Talc (Imifabi) and potato starch (Peepes) were used as excipients for dosage 100 mg ASA and 40 mg GLY, whereas microcrystalline cellulose (JRS Pharma) and maize starch (Roquette) were used as excipients for dosage 150 mg ASA and 40 mg GLY as well as 75 mg ASA and 40 mg GLY. Acetonitrile—gradient grade (Sigma-Aldrich) and orthophosphoric acid (Chempur). Deionized water was obtained by means of ELGA Purelab UHQ PS (High Wycombe, Bucks, UK). Syringe nylon filters (0.45 µm) (Agilent Technologies) were used.

Free full text: Click here

Kowalska M., Woźniak M., Kijek M., Mitrosz P., Szakiel J, & Turek P. (2022). Management of validation of HPLC method for determination of acetylsalicylic acid impurities in a new pharmaceutical product. Scientific Reports, 12, 1.

Publication 2022

Acetonitrile—gradient grade Syringe nylon filters (0.45 µm)

Acetonitrile Excipients Maize starch Microcrystalline cellulose Nylon Orthophosphoric acid Pharmaceutical Potato Salicylic acid Starch Syringe Talc

Top 5 similar protocols

Protocol cited in 79 other protocols

Variable analysis

independent variables

Dosage of acetylsalicylic acid (ASA)
Dosage of glycine (GLY)
Excipients used for different dosage formulations

dependent variables

Not explicitly mentioned

control variables

Pharmaceutical secondary standards (Certified Reference Material) of acetylsalicylic acid, salicylic acid and glycine
Syringe nylon filters (0.45 µm)
Deionized water obtained by ELGA Purelab UHQ PS
Acetonitrile—gradient grade
Orthophosphoric acid

positive controls

Pharmaceutical secondary standards (Certified Reference Material) of acetylsalicylic acid, salicylic acid and glycine

negative controls

Not explicitly mentioned

Annotations

Based on most similar protocols

The protocols use certified reference materials (CRMs) of pharmaceutical standards for calibration and validation of analytical methods (Protocol 1, Protocol 2). This helps ensure the accuracy and reliability of the results.

Ultra-pure water with high resistivity and low total organic carbon (TOC) content is used in the protocols to minimize potential contamination (Protocol 1).

The protocols utilize a variety of excipients, such as microcrystalline cellulose, maize starch, talc, and potato starch, which are used as formulation components (Input Protocol).

The protocols use HPLC-grade solvents, such as acetonitrile, methanol, and ethanol, to ensure chromatographic separation and detection (Protocol 1, Protocol 2).

Syringe nylon filters with a pore size of 0.45 μm are used to filter samples prior to analysis, helping to remove any particulate matter (Input Protocol).

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!