EdU Assay for Proliferating Cells

The EdU assay was conducted according to previous study [40 (link)]. The cells were pre-inoculated in a 24-well plate at a density of 5 × 10³ cells per well. The plated cells were then incubated in 4% methanol for 30 min, followed by permeabilization in 0.5% TritonX-100 for 10 min, and a 30-min reaction in 400 μL of 1× ApollorR. Afterward, the cells were stained with 4ʹ,6-diamidino-2-phenylindole for another 30 min. The EdU-stained cells were counted under a fluorescence microscope (CKX41-F32FL, Olympus, Tokyo, Japan) to calculate the percentage of EdU-positive cells.

Free full text: Click here

Zeng L., Yuan S., Zhou P., Gong J., Kong X, & Wu M. (2021). Circular RNA Pvt1 oncogene (CircPVT1) promotes the progression of papillary thyroid carcinoma by activating the Wnt/β-catenin signaling pathway and modulating the ratio of microRNA-195 (miR-195) to vascular endothelial growth factor A (VEGFA) expression. Bioengineered, 12(2), 11795-11810.

Publication 2021

CKX41-F32FL

Assay Fluorescence microscope Methanol

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

Incubation time in 4% methanol
Incubation time in 0.5% TritonX-100
Incubation time in 1x ApollorR

dependent variables

Percentage of EdU-positive cells

control variables

Cell density (5 × 10^3 cells per well)
Staining time with 4',6-diamidino-2-phenylindole

controls

No positive control mentioned
No negative control mentioned

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!