Isolation and Characterization of Arborescen Alkaloid

AM was extracted from the stem bark of C. arborescens, wild trees growing in Malaysia. A voucher specimen was deposited at the Herbarium, Department of Biology, University Putra Malaysia. Hexane, chloroform and methanol extracts were collected. The characterization and identification of AM have been done using JOEL ECA-400 spectrometer and mass spectra data was recorded using a Shimadzu GCMS-QP5050 spectrometer (Sidahmed et al., 2013 (link)). The purity of AM was confirmed to be 100% by HPLC-LC 20 AD (Shimadzu, Japan) using reversed-phase C18, 2.5 mm column from Waters with 10% acetonitrile in water. One peak has been observed between 7–7.5 min. The detection of the peak was at 245 nm wavelength using a Gilson absorbance detector (UV–VIS165 Gilson USA).

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El habbash A.I., Mohd Hashim N., Ibrahim M.Y., Yahayu M., Omer F.A., Abd Rahman M., Nordin N, & Lian G.E. (2017). In vitro assessment of anti-proliferative effect induced by α-mangostin from Cratoxylum arborescens on HeLa cells. PeerJ, 5, e3460.

Publication 2017

GCMS-QP5050 spectrometer HPLC-LC 20 AD

Acetonitrile Ad 20 Chloroform Gcms Hexane Hplc Mass spectra Methanol Stem Trees

Corresponding Organization :

Other organizations : University of Malaya, University of Technology Malaysia, Universiti Sains Islam Malaysia, Universiti Putra Malaysia

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Variable analysis

independent variables

Extraction solvents (hexane, chloroform, methanol)

dependent variables

Characterization and identification of AM using JOEL ECA-400 spectrometer and mass spectra data from Shimadzu GCMS-QP5050 spectrometer
Purity of AM confirmed by HPLC-LC 20 AD (Shimadzu, Japan) using reversed-phase C18, 2.5 mm column from Waters with 10% acetonitrile in water

control variables

Stem bark of C. arborescens from wild trees growing in Malaysia
Voucher specimen deposited at the Herbarium, Department of Biology, University Putra Malaysia

positive controls

Not specified

negative controls

Not specified

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