Multilocus sequence typing (MLST) was performed by sequencing seven house-keeping genes of C. difficile (adk, atpA, dxr, glyA, recA, sodA and tpi) as previously reported by Griffiths D et al. [11 (link)]. Sequence types (STs) and clades of C. difficile strains were confirmed by querying on http://pubmlst.org/ website. A minimum spanning tree generated from BioNumerics version 7.6 was used to show the genetic diversity of the MLST data derived from this study.
Capillary gel electrophoresis-based PCR ribotyping was implemented according to a previous report by Fawley WN et al. [17 (link)]. Ribotypes were identified by querying on the WEBRIBO web-based database (http://webribo.ages.at ). The novel ribotype was named as “Chongqing Ribotype” (CQR) plus two Arabic numbers (e.g., CQR01).
Capillary gel electrophoresis-based PCR ribotyping was implemented according to a previous report by Fawley WN et al. [17 (link)]. Ribotypes were identified by querying on the WEBRIBO web-based database (
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