Mice were anesthetized and positioned in a cradle that was inserted into a 30 cm horizontal bore Bruker Biospin magnet operating at 7 Tesla (Bruker BioSpin GmbH, Karlsruhe, Germany). All MRI experiments were carried out using the Bruker BA6 gradient set and mouse head coil, as previously described [14 (link)]. All animals were imaged every 3–4 days until the end of the study starting at 10 days post-G55 implantation surgery.
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