Quantitative PCR for Telomere Length

For a subset of Cross-Center samples (Penn, n = 101 and OSU, n = 111), LTL was also measured using the quantitative PCR method developed by Cawthon, modified for compatibility with the Applied Biosystems 7900 HT instrument [30 (link)](S2 Protocol). Assays were carried out in triplicate, and center samples were batch analyzed to minimize inter-assay variation. The T/S ratios of each experimental sample relative to the reference sample were generated using the comparative CT (cycle threshold) method[30 (link)]. T/S ratios and LTL kb were compared for quality control comparisons.

Free full text: Click here

Lynch S.M., Peek M.K., Mitra N., Ravichandran K., Branas C., Spangler E., Zhou W., Paskett E.D., Gehlert S., DeGraffinreid C., Rebbeck T.R, & Riethman H. (2016). Race, Ethnicity, Psychosocial Factors, and Telomere Length in a Multicenter Setting. PLoS ONE, 11(1), e0146723.

Publication 2016

7900 HT instrument

Assay

Corresponding Organization : Old Dominion University

Top 5 similar protocols

Variable analysis

independent variables

Center (Penn, OSU)

dependent variables

Telomere length (T/S ratio)
Telomere length (LTL kb)

control variables

Quantitative PCR method developed by Cawthon, modified for compatibility with the Applied Biosystems 7900 HT instrument
Assays carried out in triplicate
Center samples batch analyzed to minimize inter-assay variation

controls

Reference sample used to generate T/S ratios of each experimental sample

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!