resazurin reduction was employed as a measure of bacterial metabolic activity (59 (link), 60 (link)). Metabolites accumulating during bacterial growth reduce the weakly fluorescent resazurin to the highly fluorescent resorufin. Samples were prepared as described above but with resazurin (Sigma) added to the assay buffer to obtain a final concentration of 0.01% resazurin (w/v). Relative fluorescent units (RFU) were measured every 3 h with a preheated Victor X3 Plate Reader (Perkin Elmer) at 530 nm excitation and 616 nm emission wavelength and a top read.
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