Western Blot Analysis of Stress Proteins

Western blot analyses were performed using a previously published method [15 (link)]. The following antibodies were used: rabbit Hsp27 antibody (Assay Designs, Villeurbanne, France, 1/5000), rabbit anti-eIF4E antibody (Cell Signaling, Ozyme, 1/1000, Saint-Cyr-l’école, France), anti-rabbit IgG HRP conjugate antibody (Santa Cruz Biotechnology, Heidelberg, Germany, 1/5000), and anti-rabbit Trueblot IgG HRP conjugate antibody (eBiosciences, 1/1000, Villebon-sur-Yvette, France). Loading levels were normalized using mouse anti-vinculin antibody (Sigma-Aldrich, 1/2000, Saint-Quentin-Fallavier, France). Re-blot Plus Mild Solution (Millipore, Molsheim, France) was used for membrane stripping during 9 min at RT.

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Ziouziou H., Paris C., Benizri S., Le T.K., Andrieu C., Nguyen D.T., Appavoo A., Taïeb D., Brunel F., Oueslati R., Siri O., Camplo M., Barthélémy P, & Rocchi P. (2021). Nucleoside-Lipid-Based Nanoparticles for Phenazine Delivery: A New Therapeutic Strategy to Disrupt Hsp27-eIF4E Interaction in Castration Resistant Prostate Cancer. Pharmaceutics, 13(5), 623.

Publication 2021

rabbit anti-eIF4E antibody anti-rabbit IgG HRP conjugate antibody anti-rabbit Trueblot IgG HRP conjugate antibody mouse anti-vinculin antibody Re-blot Plus Mild Solution

Anti antibody Antibodies Antibody Assay Eif4e Hsp27 Igg hrp Membrane Mouse Rabbit Vinculin Western blot analyses

Corresponding Organization :

Other organizations : Centre de Recherche en Cancérologie de Marseille, Régulations Naturelle et Artificielle, University of Carthage, Centre Interdisciplinaire de Nanoscience de Marseille

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