Protein Content Determination by Kjeldahl Method

The protein content was calculated from the total nitrogen determined by Kjeldhal method, based on Roda and Lambri [25 (link)] and using 6.25 as the conversion factor according to AOAC method 970.22 [26 ]. Nitrogen content was determined using the digestion block and steam distillation (Tecator 2020 Digestor, Foss, Hillerød, Denmark) with colorimetric endpoint detection (Kjeltec 2300 Analyzer Unit, Foss, Denmark). Samples were evaluated in duplicate and expressed as a mass percentage.

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Monteiro S., Dias J., Lourenço V., Partidário A., Lageiro M., Lampreia C., Fernandes J., Lidon F., Reboredo F, & Alvarenga N. (2023). Development of a Functional Dark Chocolate with Baobab Pulp. Foods, 12(8), 1711.

Publication 2023

2300 Analyzer Unit

Colorimetric Digestion Distillation Foss Nitrogen Protein Steam

Corresponding Organization : Instituto Nacional de Investigação Agrária e Veterinária

Other organizations : Instituto Politécnico de Beja

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Variable analysis

independent variables

Nitrogen content

dependent variables

Protein content

control variables

Conversion factor of 6.25 according to AOAC method 970.22

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