For the characterization of nanobioconjugates, we used X-ray diffraction (XRD), Fourier transform infrared spectroscopy (FT-IR), high-resolution transmission electron microscopy (HR-TEM) and dynamic light scattering (DLS) techniques, presented in detail in our previous reference [18 (link)].
XRD was used to determine the crystalline phases and the average size of the nanocrystallites. A Rigaku UltimaIV Diffractometer with Cu Kα radiation was used for this.
FT-IR was used for the study of ferrite formation and the determination of specific Me–O bonds (Me: metal, O: oxygen) in the magnetite structure. A Shimadzu IR Affinity-1S spectrophotometer was used in the 400–4000 cm−1 range.
The morphology of the sample, and the size and distribution of the nanoparticles was studied via HR-TEM, using a Hitachi TEM system (HT7700) with 0.2 nm resolution.
Using the Vasco Particle Size Analyzer, the average hydrodynamic diameter of the nanobioconjugates in dispersion and their distribution was determined using DLS.
XRD was used to determine the crystalline phases and the average size of the nanocrystallites. A Rigaku UltimaIV Diffractometer with Cu Kα radiation was used for this.
FT-IR was used for the study of ferrite formation and the determination of specific Me–O bonds (Me: metal, O: oxygen) in the magnetite structure. A Shimadzu IR Affinity-1S spectrophotometer was used in the 400–4000 cm−1 range.
The morphology of the sample, and the size and distribution of the nanoparticles was studied via HR-TEM, using a Hitachi TEM system (HT7700) with 0.2 nm resolution.
Using the Vasco Particle Size Analyzer, the average hydrodynamic diameter of the nanobioconjugates in dispersion and their distribution was determined using DLS.
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