Transcriptional Profiling of HDF Cells

For each drug treatment, HDFs were seeded on a 96-well plate and grown for 24 h. The cells were transduced using each lentiviral supernatant for 48 h before treatment with NecB. Cells were then treated with NecB at the indicated concentrations for 10 days. Fluorescence was evaluated every 12 h using a multiple plate reader (TriStar² S LB 942, Berthold technologies, Bad Wildbad, Germany), excited at 480 nm and detected at 510 nm. The light emission was normalized by that determined in blank non-treated group and the transcription factor activity was evaluated. The regulatory networks of transcription factor activation were schematized using BTNET (http://ibtnet.korea.ac.kr/), as described previously [66 (link)].