Podophyllotoxin, as starting material, was obtained from resin of rhizome of Podophyllum peltatum as previous described [16 (link)]. Further transformation into podophyllic aldehyde was conducted following the synthetic procedure described previously by us [15 (link)]. Etoposide was purchased from Sigma-Aldrich (#E1383, Saint Louis, MO, USA). Compounds were dissolved in DMSO (10−2 M) and further dilutions were performed in PBS Na+/K+. Final concentration of DMSO in assays were <0.001%.
Dimethyl sulfoxide (DMSO) was obtained from Merck (Darmstadt, Germany). Bovine serum albumin (BSA), trypan blue, urea, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and 96-well plates and BCA protein assay kit were purchased from Thermo Scientific (Rockford, IL, USA). Heat inactivated fetal bovine serum (FBS), L-glutamine, penicillin–streptomycin (P-S), and 0.25% trypsin-EDTA were purchased from Gibco® (Gran Island, NY, USA), and 6-well, clear, flat-bottom plates were purchased from Corning (Corning, NY, USA). Cycloscope™ reagent for cell cycle analysis was purchased from Cytognos (Salamanca, Spain).
Dimethyl sulfoxide (DMSO) was obtained from Merck (Darmstadt, Germany). Bovine serum albumin (BSA), trypan blue, urea, and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) were purchased from Sigma-Aldrich (St. Louis, MO, USA), and 96-well plates and BCA protein assay kit were purchased from Thermo Scientific (Rockford, IL, USA). Heat inactivated fetal bovine serum (FBS), L-glutamine, penicillin–streptomycin (P-S), and 0.25% trypsin-EDTA were purchased from Gibco® (Gran Island, NY, USA), and 6-well, clear, flat-bottom plates were purchased from Corning (Corning, NY, USA). Cycloscope™ reagent for cell cycle analysis was purchased from Cytognos (Salamanca, Spain).
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