Lignin Auto-Fluorescence Microscopy

Lignin production was recorded by the auto-fluorescence microscopy, as described in a previous study [65 (link)]. According to the author, lignin has a wide array of fluorescence emission and is excited by UV rays. Lignified cell walls of xylem tissue show auto-fluorescence. Thin hand sections of leaf petiole of different treated leaves were made with a fine blade and mounted in 50% glycerol. Auto-fluorescence of lignin was visualized with the help of a Floid Cell Imaging Station microscope (Life Technologies) under the excitation wavelength 330 nm to 380 nm and emission wavelength between 500 and 530 nm.