qRT-PCR Analysis of Mouse Prefrontal Cortex

For the qRT-PCR analyses, the prefrontal cortex (PFC) tissues from mice were collected immediately following decapitation under anesthesia, according to a mouse brain atlas [21 (link)]. Total RNA from the PFC samples was isolated by using a commercially available kit (SV RNA Isolation, Promega, Madison, WI, USA). MasterCycler (Eppendorf, Westburg, NY, USA) was used to perform qRT-PCR using iTaq^TM Universal SYBR® Green Supermix (Bio-Rad, CA, USA). Primers specific to genes were synthesized by Integrated DNA Technologies. Housekeeping gene (beta2-microglobulin (B2m)) was used to normalize the gene of interest. A list of primers used is given in Table S1.