Reagents and Vectors for SLC7A11 and G6PD Studies

SLC7A11 cDNA-containing expression vectors were described in previous publications ^{14 (link), 22 (link), 43 (link)}. G6PD shRNA constructs in GIPZ vector and G6PD cDNA were obtained from the Functional Genomics Core Facility of The University of Texas MD Anderson Cancer Center. G6PD cDNA was subsequently cloned into the lentivirus vector pCDH-hygromycin with C-terminal Myc tag. All constructs were confirmed by DNA sequencing. D-[1, 2-¹³C2] glucose (#GLC-026), 2-deoxy-D-[1-²H] glucose, and D-[³-2H] glucose (#GLC-034) were obtained from Omicro Biochemical. L-[1, 2, 1’, 2’−14C]-Cystine (#NEC854010UC) was from Perkine Elmer. Epiandrosterone (#CS-5183) was obtained from Chemscene. Following reagents were obtained from Sigma: BAY-876 (#SML1774–25MG), L-Glutamine-13C5 (#605166–100MG), N-Acetyl-L-Cysteine (NAC) (#A9165), 2-Deoxy-D-glucose (#D8375–1G), Sulfasalazine (#S0883–10G), D-Penicillamine (#P4875), L-Penicillamine (#196312), Trolox (#238813), 4-Hydroxy-TEMPO (#176141), Tris(2-carboxyethyl)phosphine hydrochloride(TCEP) (#C4706), 2-Mercaptoethanol (2ME) (#M6250), Deferoxamine mesylate salt (DFO) (#D9533), Ferrostatin-1 (#SML0583), 6-Aminonicotinamide (#A68203–1G), Methyl cellulose (#M0512–100G), TWEEN 80 (# P1754–500ML). All reagents were dissolved according to manufacturers’ instructions.

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Liu X., Olszewski K., Zhang Y., Lim E.W., Shi J., Zhang X., Zhang J., Lee H., Koppula P., Lei G., Zhuang L., You M.J., Fang B., Li W., Metallo C.M., Poyurovsky M.V, & Gan B. (2020). Cystine transporter regulation of pentose phosphate pathway dependency and disulfide stress exposes a targetable metabolic vulnerability in cancer. Nature cell biology, 22(4), 476-486.

Publication 2020

Epiandrosterone BAY-876 L-Glutamine-13C5 N-Acetyl-L-Cysteine (NAC 2-Deoxy-D-glucose Sulfasalazine D-Penicillamine L-Penicillamine Trolox 4-Hydroxy-TEMPO 2-Mercaptoethanol Deferoxamine Ferrostatin-1 6-Aminonicotinamide Methyl cellulose TWEEN 80

2 mercaptoethanol 4 hydroxy tempo 6 aminonicotinamide Bay 876 Cancer Cdna Cystine Deferoxamine mesylate Deoxy Epiandrosterone Ferrostatin 1 G6pd Glucose Hygromycin L glutamine Lentivirus Methyl cellulose N acetyl l cysteine Penicillamine Phosphine Salt Shrna Sulfasalazine Tcep Tris Trolox Tween 80 Vector

Corresponding Organization :

Other organizations : The University of Texas MD Anderson Cancer Center, Eon Corporation (United States), University of California, San Diego, Baylor College of Medicine, The University of Texas Health Science Center at Houston

Top 5 similar protocols

Variable analysis

independent variables

SLC7A11 cDNA-containing expression vectors
G6PD shRNA constructs in GIPZ vector
G6PD cDNA cloned into the lentivirus vector pCDH-hygromycin with C-terminal Myc tag
D-[1, 2-¹³C2] glucose
2-deoxy-D-[1-²H] glucose
D-[³-2H] glucose
L-[1, 2, 1', 2'−14C]-Cystine
Epiandrosterone
BAY-876
L-Glutamine-13C5
N-Acetyl-L-Cysteine (NAC)
2-Deoxy-D-glucose
Sulfasalazine
D-Penicillamine
L-Penicillamine
Trolox
4-Hydroxy-TEMPO
Tris(2-carboxyethyl)phosphine hydrochloride (TCEP)
2-Mercaptoethanol (2ME)
Deferoxamine mesylate salt (DFO)
Ferrostatin-1
6-Aminonicotinamide
Methyl cellulose
TWEEN 80

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

positive controls

None mentioned

negative controls

None mentioned

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