Analytical Characterization of Marine Fucoidans

Fucus vesiculosus fucoidan (FVF, batch No. DPFVF2021001) and Undaria pinnatifida fucoidan (UPF; batch No. DPGFS2019537) were provided by Marinova (Cambridge, TAS, Australia). The materials were extracted using a proprietary aqueous extraction process. The fucoidan purity of both samples was >95% (dry weight). The fucoidan component and compositional profile for each material can be found in Table 1. The calculation of fucoidan purity requires several inputs that are determined using a range of spectrophotometric assays. The total carbohydrate content of a hydrolyzed sample was assessed using the phenol–sulfuric technique developed by [57 (link)], while the uronic acid concentration was determined in the presence of 3-phenylphenol, based on the method by [58 (link)]. Sulfate content was analyzed using a BaSO₄ precipitation method [59 (link)]. UPF and FVF were dissolved in saline solution to perform the assays.