The type of interaction derived from MTF/PyP and PMM/PyP when they are in binary combination was described by the median-effect/combination index (CI)-isobologram equation [33 (link),34 (link)]. The CI < 1, = 1, and > 1 shows synergism, additive and antagonism effect of the combination, respectively. These interactions were analyzed with CalcuSyn software version 2.1. (Biosoft, Cambridge, UK).
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