Agar (A) powder was purchased from Zhenpai Hydrocolloids Co., Ltd. (Zhangzhou City, Fujian Province, China), with moisture 7.97 wt%, pH 6.46, and 1.5% gel strength of 1150 g/cm2. FP was isolated from the leaves of E. angustifolium L. by the treatment of plant raw materials with aqueous hydrochloric acid at pH 0.8 [20 (link)]. The PC content in FP was 27 mg GAE/g. The chemical characteristics of the polysaccharides are shown in Table 5 .
The reagents, including the Folin and Ciocalteu’s phenol reagent and pectinase from Aspergillus niger (1.18 U/mg), were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gallic acid was purchased from MP Biomedicals (Solon, OH, USA). Ethanol (95%, JSC Kirov Pharmaceutical Factory, Kirov, Russia), pyridine, boric acid, toluene and chloroform stabilized with ethyl alcohol (Vekton, Saint Petersburg, Russia), methanol (Merck, München, Germany, 99.5%), 3,5-dimethylphenol (Sigma Aldrich, USA, 99%), sodium borohydride (Sigma Aldrich, USA, 98.5%), Sulphuric acid (Sigma-tec, Moscow, Russia) were used.
The reagents, including the Folin and Ciocalteu’s phenol reagent and pectinase from Aspergillus niger (1.18 U/mg), were purchased from Sigma-Aldrich (St. Louis, MO, USA). Gallic acid was purchased from MP Biomedicals (Solon, OH, USA). Ethanol (95%, JSC Kirov Pharmaceutical Factory, Kirov, Russia), pyridine, boric acid, toluene and chloroform stabilized with ethyl alcohol (Vekton, Saint Petersburg, Russia), methanol (Merck, München, Germany, 99.5%), 3,5-dimethylphenol (Sigma Aldrich, USA, 99%), sodium borohydride (Sigma Aldrich, USA, 98.5%), Sulphuric acid (Sigma-tec, Moscow, Russia) were used.
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