A transwell migration assay was conducted as per our previous study (30 (link)). Briefly, cells in the logarithmic growth phase were seeded in the upper transwell chamber insert (Corning, USA) at a density of 2×104 cells per well. To inhibit cell division, serum-free medium containing 1 mM mitomycin was used to replace the complete medium. A 24-well plate was used to place the chamber, where the upper chamber containing FBS-free medium and the lower chamber containing 10% FBS complete medium. Cells cultured for 24 hours. Then discarded the medium and stained the cells with 1% crystal violet solution in order to present the amount of migrated cells.
Protocol detail
Transwell Migration Assay Protocol
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Corresponding Organization :
Other organizations : Fujian Medical University, First Affiliated Hospital of Xiamen University, Fujian Provincial Hospital
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Variable analysis
independent variables
- Cell seeding density (2×10^4 cells per well)
- Amount of migrated cells
- Growth phase of cells (logarithmic)
- Culture time (24 hours)
- Culture medium (serum-free medium containing 1 mM mitomycin in the upper chamber, 10% FBS complete medium in the lower chamber)
- Transwell chamber (Corning, USA)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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