Before subjected to heat shock, the ISRIB treatment group cells were pre-treated with 200 nM ISRIB (Merk) for 30 minutes. After heat shock, 100 nM epoxomicin (Sigma) was added for 4 hours. To test the effect of ISRIB on sodium arsenate-induced stress, cells were first pretreated with 200 nM ISRIB for 30 min followed by incubation with 50 µM sodium arsenate (Sigma) for 4 hours. For the proteasome inhibitor titration experiments, Ub-YFP MelJuSo cells were treated with the indicated epoxomicin concentration for 4 hours.
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Heat Shock and Proteasome Inhibition
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Corresponding Organization : Karolinska Institutet
Other organizations : Center for Systems Biology Dresden, TU Dresden
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Variable analysis
independent variables
- ISRIB treatment (200 nM ISRIB for 30 minutes)
- Heat shock
- Epoxomicin treatment (100 nM for 4 hours)
- Sodium arsenate treatment (50 μM for 4 hours)
- Epoxomicin concentration (titration)
- Not explicitly mentioned
- Not explicitly mentioned
- Positive control: Not specified
- Negative control: Not specified
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