Comprehensive Transcriptomic Profiling of Cancer Progression

The nCounter® PanCancer Progression panel (NanoString Technologies Inc., Seattle WA) is comprised of 740 genes related to cancer progression processes including angiogenesis, extracellular matrix remodeling (ECM), EMT, tumor growth, and metastasis. Details for the probes included in the Progression panel are available on request from NanoString Technologies Inc. using the form at http://www.nanostring.com/support-documents/ncounter-pancancer-progression-panel-gene-list/. We also designed a custom panel of 69 genes related to arylamine exposure and bladder cancer plus 30 housekeeping genes, 8 negative controls, and 6 spike-in hybridization/positive controls overlapping with those in the Progression panel. The custom genes and their annotations are listed in Supplementary Table S2. Genes in the custom panel were selected from in vitro and in vivo arylamine exposure studies [19 (link)–21 (link), 23 (link), 24 (link)] and include 25 genes related to the DNA damage signaling pathway [19 (link), 23 (link)] and 4 genes related to EMT [20 (link), 25 (link)]. We also included several genes related to bladder cancer from The Cancer Genome Atlas (TCGA) [26 (link)–28 (link)], which are highlighted in the Supplementary Table S2.

Free full text: Click here

Kolli R.T., Xu Z., Panduri V, & Taylor J.A. (2021). Differential Gene Expression in Bladder Tumors from Workers Occupationally Exposed to Arylamines. BioMed Research International, 2021, 2624433.

Publication 2021

nCounter® PanCancer Progression panel

A genes Angiogenesis Bladder cancer Cancer Dna damage Genes Genome Housekeeping genes Hybridization Metastasis Progression Several genes Signaling pathway Tumor

Corresponding Organization : National Institute of Environmental Health Sciences

Top 5 similar protocols

Variable analysis

independent variables

Arylamine exposure

dependent variables

Gene expression related to cancer progression, DNA damage signaling, and EMT

control variables

Housekeeping genes
Negative controls
Spike-in hybridization/positive controls

controls

Positive controls: Spike-in hybridization/positive controls
Negative controls: 8 negative controls

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!