Blue-light Induction Methodology

Blue-light induction was accomplished by either (1) illuminating cultures grown in glass culture tubes on a roller drum or (2) in a Light Plate Apparatus (LPA) (Gerhardt, et al., 2016 ). Light intensity was measured and validated using a standard photodiode power sensor and power meter (Thorlabs #S120VC, Thorlabs #PM100D). The LPA was calibrated as described in Sweeney, et al (Sweeney, Moreno Morales, Burmeister, Nimunkar, & McClean, 2019 (link)) so that consistent light doses could be delivered between LPAs and between experiments.

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An-adirekkun J.(., Stewart C.J., Geller S.H., Patel M.T., Melendez J., Oakes B.L., Noyes M.B, & McClean M.N. (2019). A yeast optogenetic toolkit (yOTK) for gene expression control in Saccharomyces cerevisiae. Biotechnology and bioengineering, 117(3), 886-893.

Publication 2019

S120VC

Light

Corresponding Organization : University of Wisconsin–Madison

Other organizations : Princeton University, Institute for Systems Biology

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Variable analysis

independent variables

Method of blue-light induction (illuminating cultures in glass culture tubes on a roller drum or in a Light Plate Apparatus (LPA))

dependent variables

Light intensity

control variables

Consistent light doses between LPAs and between experiments, as the LPA was calibrated as described in Sweeney, et al. (2019)

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