All animal experiments were performed according to procedures approved by Beth Israel Deaconess Medical Center’s Institutional Animal Care and Use Committee. The mice (strain: B6.Cg-Lepob/J from Jackson Labs) were 8 weeks old when administered inhibitor or vehicle by once daily oral gavage for 5 days. Doses in mice (GSK1120212, 3 mg/kg in DMSO; PD0325901, 10 mg/kg in DMSO) were diluted in 15% Cremophor EL/ 82% saline/ 3% drug or DMSO were comparable to that given in human clinical trials [5 (link), 9 (link), 10 (link), 14 (link)]. Kidneys, livers, and pancreata from nine mice were dissected and flash frozen in liquid nitrogen.
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Evaluating Kinase Inhibitors in Obese Mice
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Cremophor el
Dmso
Drug
Frozen
Gavage
Gsk1120212
Human
Institutional animal care and use committee
Kidneys
Livers
Mice
Nitrogen
Pancreata
Pd0325901
Saline
Strain
Corresponding Organization : Harvard University
Other organizations : Brigham and Women's Hospital
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Variable analysis
independent variables
- Inhibitor (GSK1120212) or vehicle (15% Cremophor EL/ 82% saline/ 3% DMSO)
- Inhibitor (PD0325901) or vehicle (15% Cremophor EL/ 82% saline/ 3% DMSO)
- Kidneys, livers, and pancreata from nine mice (dissected and flash frozen in liquid nitrogen)
- Mouse strain: B6.Cg-Lepob/J from Jackson Labs
- Mouse age: 8 weeks old
- Route of administration: Once daily oral gavage for 5 days
- Doses in mice: GSK1120212 (3 mg/kg in DMSO), PD0325901 (10 mg/kg in DMSO)
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