Coefficient of Variation (c V) was calculated using FinCal v0.6.3 (55 ). Differential gene expression analysis was performed with limma package (49 (link)). Permutational multivariate analysis of variance (PERMANOVA) was used to compare distances between gene expression profiles of CL lesions and healthy skin. Receiver operating characteristic (ROC) curves were made with plotROC package to calculate area under the curve (AUC) for potential markers of treatment outcome (56 (link)). Mann-Whitney U tests and Spearman’s rho (ρ) correlations were performed in the R statistical environment or GraphPrism v7, and P<0.05 was considered statistically significant.
Protocol detail
Differential Gene Expression Analysis
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Gene expression analysis
Skin
Corresponding Organization : University of Pennsylvania
Other organizations : Complexo Hospitalar Universitário Professor Edgard Santos, Universidade Federal da Bahia, Fundação Oswaldo Cruz
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Variable analysis
independent variables
- Not explicitly mentioned
- Gene expression profiles of CL lesions and healthy skin
- Potential markers of treatment outcome
- Not explicitly mentioned
- Positive controls: Not mentioned
- Negative controls: Not mentioned
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