Multicolor Flow Cytometry Analysis of Immune Cell Subpopulations

Whole blood samples of the patients were collected and the fresh whole blood was analyzed by multicolor ﬂow cytometry according to our previously published and optimized IPT protocols.17 18 (link) IPT was performed within 3 hours after the collection of whole blood. Data acquisition was performed on a Gallios Flow Cytometer (Beckman Coulter) in the standard filter configuration. The Kaluza Flow Analysis Software (Beckman Coulter) was used for data analysis. The immune cell subpopulations analyzed are visualized and specified in online supplemental sFigure 1.

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Zhou J.G., Donaubauer A.J., Frey B., Becker I., Rutzner S., Eckstein M., Sun R., Ma H., Schubert P., Schweizer C., Fietkau R., Deutsch E., Gaipl U, & Hecht M. (2021). Prospective development and validation of a liquid immune profile-based signature (LIPS) to predict response of patients with recurrent/metastatic cancer to immune checkpoint inhibitors. Journal for Immunotherapy of Cancer, 9(2), e001845.

Publication 2021

Gallios Flow Cytometer Kaluza Flow Analysis Software

Blood Cell Patients Subpopulations

Corresponding Organization : Universitätsklinikum Erlangen

Other organizations : Zunyi Medical University, Université Paris-Saclay, Inserm, CentraleSupélec

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Immune cell subpopulations

control variables

Fresh whole blood samples
Multicolor flow cytometry analysis
IPT protocols performed within 3 hours of blood collection

controls

Positive control: Not specified
Negative control: Not specified

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