Quantifying ESR1 Protein Levels in Pituitaries

Western blotting for ESR1 was performed as reported previously [26 (link)] on d100 control (n = 4) and Foxg1^Cre/+; AR^fl/y (n = 4) pituitaries. Blots were probed with primary antibodies ESR1 (Santa Cruz sc-542) and tyrosinated TUBA (α-tubulin) isoforms (Abcam ab6160). Primary antibodies were detected using donkey anti-rabbit IRDye 680RD and goat anti-rat IRDye 800CW (LI-COR Biosciences). Detection was carried out using the LI-COR Odyssey imaging system (LI-COR biosciences) according to manufacturer’s instructions.

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O’Hara L., Curley M., Tedim Ferreira M., Cruickshanks L., Milne L, & Smith L.B. (2015). Pituitary Androgen Receptor Signalling Regulates Prolactin but Not Gonadotrophins in the Male Mouse. PLoS ONE, 10(3), e0121657.

Publication 2015

sc-542 ab6160 IRDye 680RD IRDye 800CW LI-COR Odyssey imaging system

Antibodies Donkey Goat Irdye 800cw Isoforms Pituitaries Rabbit α tubulin

Corresponding Organization :

Other organizations : MRC Centre for Reproductive Health, Medical Research Council, University of Edinburgh

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Variable analysis

independent variables

Genotype: Control (d100 control) vs. Foxg1^Cre/+; AR^fl/y

dependent variables

Protein levels of ESR1 (Estrogen Receptor 1)

control variables

Age of pituitaries (d100)

controls

Positive control: Not specified
Negative control: Not specified

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