Histone Modifications Profiling by ChIP

Mouse monoclonal antibodies against the following histone modifications were used: H3K9me2 (m5.1.1) (36 (link)), H3K36me2, H3K36me3, H3K9Ac and H3K14Ac (37 (link),38 (link)). Mouse monoclonal antibodies against epitope tags, i.e. Myc (4A6, Millipore) and FLAG (M2, Sigma), were used in the ChIP assay and immunoblotting. Mouse monoclonal antibody against Pol2 CTD phosphorylated at Ser₅ (4H8, Abcam), rabbit polyclonal antibody against the N-terminal half of Rpb1 (39 (link)) and rabbit polyclonal antibody against histone H3 C-terminal (Millipore) were used for immunoblotting.

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Suzuki S., Kato H., Suzuki Y., Chikashige Y., Hiraoka Y., Kimura H., Nagao K., Obuse C., Takahata S, & Murakami Y. (2016). Histone H3K36 trimethylation is essential for multiple silencing mechanisms in fission yeast. Nucleic Acids Research, 44(9), 4147-4162.

Publication 2016

Myc (4A6, FLAG (M2,

Antibody Chip assay Epitope Histone h3 Histone modifications Monoclonal antibody Mouse Rabbit

Corresponding Organization : Hokkaido University

Other organizations : Shimane University, Japan Science and Technology Agency, The University of Tokyo, National Institute of Information and Communications Technology, Osaka University, Tokyo Institute of Technology

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Variable analysis

independent variables

Mouse monoclonal antibodies against the following histone modifications: H3K9me2 (m5.1.1), H3K36me2, H3K36me3, H3K9Ac and H3K14Ac
Mouse monoclonal antibodies against epitope tags: Myc (4A6, Millipore) and FLAG (M2, Sigma)
Mouse monoclonal antibody against Pol2 CTD phosphorylated at Ser5 (4H8, Abcam)
Rabbit polyclonal antibody against the N-terminal half of Rpb1
Rabbit polyclonal antibody against histone H3 C-terminal (Millipore)

dependent variables

Not explicitly mentioned

control variables

Not explicitly mentioned

controls

Positive control: None mentioned
Negative control: None mentioned

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