Caspase-3 immunohistochemistry was performed as previously described method [4 (link),20 (link)]. The sections were incubated overnight with a rabbit anti–caspase-3 antibody (1:500; Santa Cruz Biotechnology, Dallas, TX, USA), and then incubated for another 1 hour with the biotinylated rabbit secondary antibody. The bound secondary antibody was then amplified using a Vector Elite ABC kit (Vector Laboratories). The antibody-biotinavidin-peroxidase complex was visualized using 0.02% DAB.
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Caspase-3 Immunohistochemistry Protocol
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Corresponding Organization :
Other organizations : Korea University, Kyung Hee University, Jeju National University
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Variable analysis
independent variables
- Caspase-3 immunohistochemistry protocol
- Caspase-3 expression
- Incubation time with primary antibody
- Incubation time with secondary antibody
- Concentration of primary antibody
- Concentration of secondary antibody
- Visualization method (Vector Elite ABC kit and DAB)
- Positive control: Not explicitly mentioned
- Negative control: Not explicitly mentioned
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