Immunohistochemistry was performed on tissue microarray (TMA) sections. TMA slides were stained for FOXA1 (Abcam ab170933, 1:100 dilution with 10 mM NaCitrate antigen retrieval) and FOXA2 (Abcam ab108422, 1:500 dilution with 10 mM NaCitrate antigen retrieval) using a standard procedure67 (link). Rabbit IgG was used as a negative control. Nuclear staining intensity was assigned levels 0, 1+, 2+, or 3+ and H-scores were calculated as: [1 x (% of 1+ cells) + 2 x (% of 2+ cells) + 3 x (% of 3+ cells)]. Evaluations were performed in a blinded fashion.
Baca S.C., Takeda D.Y., Seo J.H., Hwang J., Ku S.Y., Arafeh R., Arnoff T., Agarwal S., Bell C., O’Connor E., Qiu X., Alaiwi S.A., Corona R.I., Fonseca M.A., Giambartolomei C., Cejas P., Lim K., He M., Sheahan A., Nassar A., Berchuck J.E., Brown L., Nguyen H.M., Coleman I.M., Kaipainen A., De Sarkar N., Nelson P.S., Morrissey C., Korthauer K., Pomerantz M.M., Ellis L., Pasaniuc B., Lawrenson K., Kelly K., Zoubeidi A., Hahn W.C., Beltran H., Long H.W., Brown M., Corey E, & Freedman M.L. (2021). Reprogramming of the FOXA1 cistrome in treatment-emergent neuroendocrine prostate cancer. Nature Communications, 12, 1979.
Other organizations :
Dana-Farber Cancer Institute, National Cancer Institute, Center for Cancer Research, Cedars-Sinai Medical Center, University of California, Los Angeles, University of Washington, Fred Hutch Cancer Center, Broad Institute, Eli and Edythe Broad Foundation
Nuclear staining intensity (levels 0, 1+, 2+, or 3+)
H-score (calculated as: [1 x (% of 1+ cells) + 2 x (% of 2+ cells) + 3 x (% of 3+ cells)])
control variables
Antibody dilution (FOXA1 1:100, FOXA2 1:500)
Staining procedure (standard procedure)
positive control
Tissue microarray (TMA) sections
negative control
Rabbit IgG
Annotations
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