Metagenomic Analysis of Rotten Food Waste

DNA was extracted from the rotten food waste with NucleoSpin DNA Stool kits (Macherey–Nagel GmbH & Co. KG, Düren, Germany) and quality checked by using a NanoDrop (Thermo Fisher Scientific Inc., MA, USA) and Qubit 4 Fluorometer (Thermo Fisher Scientific Inc.). Sequencing libraries were prepared with a 16S Barcoding Kit (SQK-16S024, Oxford Nanopore Technologies, UK) and sequenced by using MinION (Oxford Nanopore Technologies). The data were demultiplexed by using the adapter trimming tool Porechop^{34 (link)}, and Emu^{35 (link)} was used to estimate sequence relative abundance, with default settings. NMDS analysis was performed by using the vegan package in R³⁶.

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Michishita R., Shimoda M., Furukawa S, & Uehara T. (2023). Inoculation with black soldier fly larvae alters the microbiome and volatile organic compound profile of decomposing food waste. Scientific Reports, 13, 4297.

Publication 2023

NucleoSpin DNA Stool kits NanoDrop Qubit 4 Fluorometer 16S Barcoding Kit MinION

Food Nmds Stool Vegan

Corresponding Organization :

Other organizations : Institute of Agrobiological Sciences, The University of Tokyo, University of Tsukuba

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Variable analysis

independent variables

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dependent variables

Sequence relative abundance

control variables

No control variables explicitly mentioned

controls

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