Measuring Neuroinflammatory Markers in ART-Naïve Individuals

We selected monocyte and macrophage activation markers, as well as specific inflammatory markers that have previously been associated with NCI in ART-naïve individuals35 (link)36 (link)37 (link). Levels of selected monocyte activation and inflammatory markers were measured in CSF and blood plasma by enzyme-linked immunosorbent assay (ELISA): sCD163 (Trillium Diagnostics, Brewer, ME, USA); or by electrochemiluminescence multiplex assay: MCP-1, IL-8, IL-6 and TNF-α, (Meso Scale Diagnostics, Rockville, MD, USA), according to the manufacturer’s procedures.

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Oliveira M.F., Murrel B., Pérez-Santiago J., Vargas M., Ellis R.J., Letendre S., Grant I., Smith D.M., Woods S.P, & Gianella S. (2015). Circulating HIV DNA Correlates With Neurocognitive Impairment in Older HIV-infected Adults on Suppressive ART. Scientific Reports, 5, 17094.

Publication 2015

MCP-1 IL-6 TNF-α

Assay Blood plasma Diagnostics Enzyme linked immunosorbent assay Inflammatory Macrophage activation Mcp 1 Monocyte Tnf α Trillium

Corresponding Organization :

Other organizations : University of California, San Diego, Neurobehavioral Research (United States), VA San Diego Healthcare System, University of Houston

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Levels of selected monocyte activation and inflammatory markers in CSF and blood plasma
SCD163
MCP-1
TNF-α

control variables

None explicitly mentioned

controls

Positive controls: Not specified
Negative controls: Not specified

Annotations

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