Immunohistochemical Analysis of Adult Thymus

Adult thymi were processed for IHC as described (Nowell et al., 2011 (link)). Isotype controls (not shown) were included in all experiments. Staining was analyzed using a Leica AOBS confocal microscope (Leica Microsystems). The images presented are either single optical sections or projected focus stacks of serial optical sections. The number of FOXN1⁺ or AIRE⁺ TECs per area was established by analyzing three areas on each of two non-sequential sections from each of three individual mice. Statistical analysis was performed on the average number of FOXN1⁺ or AIRE⁺ cells per condition.

Free full text: Click here

Bredenkamp N., Nowell C.S, & Blackburn C.C. (2014). Regeneration of the aged thymus by a single transcription factor. Development (Cambridge, England), 141(8), 1627-1637.

Publication 2014

Leica AOBS confocal microscope

Adult Aire Cells Confocal microscope Foxn1 Isotype Mice Sections Tecs Thymi

Corresponding Organization :

Other organizations : MRC Centre for Regenerative Medicine, Medical Research Council, University of Edinburgh

Top 5 similar protocols

Protocol cited in 1 other protocol

Variable analysis

independent variables

None explicitly mentioned

dependent variables

Number of FOXN1+ TECs per area
Number of AIRE+ TECs per area

control variables

Tissue processing procedure (as described in Nowell et al., 2011)
Isotype controls included in all experiments
Staining analysis using a Leica AOBS confocal microscope
Number of areas analyzed per section
Number of non-sequential sections analyzed per mouse
Number of individual mice per condition

controls

Isotype controls (not shown) were included in all experiments

Annotations

Based on most similar protocols

Etiam vel ipsum. Morbi facilisis vestibulum nisl. Praesent cursus laoreet felis. Integer adipiscing pretium orci. Nulla facilisi. Quisque posuere bibendum purus. Nulla quam mauris, cursus eget, convallis ac, molestie non, enim. Aliquam congue. Quisque sagittis nonummy sapien. Proin molestie sem vitae urna. Maecenas lorem.

As authors may omit details in methods from publication, our AI will look for missing critical information across the 5 most similar protocols.

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!