Targeted Metabolite Profiling in Serum

Before analysis, all serum samples were processed as described, with samples thawed on ice, then centrifuged; the supernatant was subjected to further analyses^{6 (link),9 (link)}. The AbsoluteIDQ p180 kit assay (Biocrates Life Sciences AG, Innsbruck, Austria) permitted targeted, fully automated quantification of 188 analytes (42 amino acids or biogenic amines and their derivatives) based on phenylisothiocyanate derivatization in the presence of internal standards followed by liquid chromatography mass spectrometry using a TSQ-Vantage (Thermo Fisher Scientific, Waltham, MA) instrument with electrospray ionization.

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Michel M., Dubowy K.O., Entenmann A., Karall D., Adam M.G., Zlamy M., Odri Komazec I., Geiger R., Niederwanger C., Salvador C., Müller U., Laser K.T, & Scholl-Bürgi S. (2020). Targeted metabolomic analysis of serum amino acids in the adult Fontan patient with a dominant left ventricle. Scientific Reports, 10, 8930.

Publication 2020

AbsoluteIDQ p180 kit assay TSQ-Vantage

Amino acids Assay Biogenic amines Derivatives Liquid chromatography Mass spectrometry Phenylisothiocyanate Serum

Corresponding Organization : Universität Innsbruck

Other organizations : Heart and Diabetes Center North Rhine-Westphalia, Biocrates Life Sciences (Austria)

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Variable analysis

independent variables

None explicitly mentioned

dependent variables

Quantification of 188 analytes (42 amino acids or biogenic amines and their derivatives)

control variables

Serum samples were processed as described, with samples thawed on ice, then centrifuged
The supernatant was subjected to further analyses
The AbsoluteIDQ p180 kit assay was used for targeted, fully automated quantification of the analytes
Phenylisothiocyanate derivatization in the presence of internal standards was performed
Liquid chromatography mass spectrometry using a TSQ-Vantage (Thermo Fisher Scientific, Waltham, MA) instrument with electrospray ionization was used

positive controls

None explicitly mentioned

negative controls

None explicitly mentioned

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