Multiplex Immunofluorescence Staining for TMAs

The multiplex quantitative immunofluorescence staining for TMA slides was performed as previously described.14–17 (link) Slides were fluorescently stained with Opal 7-Color Manual IHC Kit (NEL811001KT) according to the manufacturer’s description. Multispectral images of arrays were acquired using Vectra Polaris multispectral imaging system (PerkinElmer), and quantitative positivity of primary antibodies was analyzed using inForm Tissue Finder software (PerkinElmer).

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Liu F., Liu W., Zhou S., Yang C., Tian M., Jia G., Wang H., Zhu B., Feng M., Lu Y., Qiao T., Wang X., Cao W., Wang X., Shi Y, & Wu D. (2020). Identification of FABP5 as an immunometabolic marker in human hepatocellular carcinoma. Journal for Immunotherapy of Cancer, 8(2), e000501.

Publication 2020

Vectra Polaris multispectral imaging system inForm Tissue Finder software

Antibodies Immunofluorescence Opal Tissue Vectra

Corresponding Organization :

Other organizations : Clinical Science Institute, Fudan University, Zhongshan Hospital, Second Affiliated Hospital of Fujian Medical University, Fujian Medical University, Guangzhou Medical University

Top 5 similar protocols

Variable analysis

independent variables

Multiplex quantitative immunofluorescence staining protocol

dependent variables

Quantitative positivity of primary antibodies

control variables

Tissue microarray (TMA) slides
Opal 7-Color Manual IHC Kit (NEL811001KT)
Vectra Polaris multispectral imaging system (PerkinElmer)
InForm Tissue Finder software (PerkinElmer)

controls

Positive control: Not explicitly mentioned
Negative control: Not explicitly mentioned

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